Prof.
Christoph Borchers from from McGill University, Montreal Canada who works in the Division of Experimental Medicine will present on
Quantitative Proteomics – past, present, and future
Early untargeted proteomics methods, such as MALDI-MS and LC/MS/MS, provided only information on protein identification. Various “bottom-up” methods were then developed, using the peak heights of component peptides to provide relative quantitation. In ~2005, targeted quantitative methods – based on the use of isotopically labelled internal standards peptides (SIS peptides)–started becoming more widely used, and quantitative proteomics became more precise and reproducible between laboratories. The tandem mass spectrometry technique known as “multiple reaction monitoring”, where the transition from a precursor to a fragment ion is measured, has enabled a steady increase in the number of protein targets from a single target to the multiplexed quantitation of 100s of protein targets in a single analysis. The newest method we are currently developing is called “SysQuan” – this method uses tissue from a labelled mouse as a source of biologically generated internal standard peptides. Using this approach, it is now possible to do untargeted protein quantitation of 1000s of proteins in a single analysis. Using this breakthrough approach, we are working toward the quantitation of an entire proteome in a single analysis.
Dr. Christoph Borchers is recognized as a pioneer and leading figure in the development of mass spectrometry-based methods for protein quantification using Multiple Reaction Monitoring (MRM) and structural proteomics. He has published more than 350 peer-reviewed papers which have been cited more than 30,000 times (h-factor 82). He is the founder and director of the McGill-Lady Davis Institute Integrated Proteomics Program at the Jewish General Hospital, McGill University, where he is currently a Full Professor in the Gerald Bronfman Department of Oncology. Dr. Borchers received his PhD degree from the University of Konstanz, Germany in 1996. After his post-doctoral training at the NIEHS/NIH/RTP, NC he became the director of the UNC-Duke Proteomics Facility and held a faculty position at the UNC Medical School in Chapel Hill, NC (2001-2006). From 2006 to 2019, he was a Professor in the Department of Biochemistry & Microbiology, and Director of the University of Victoria-Genome BC Proteomics Centre at the University of Victoria, BC, Canada, where he held the Rix BC Leadership Chair in Biomedical and Environmental Proteomics.
Friday, November 1st, 2024, 3:30 PM, Bertrand Snell Hall 214Potsdam Campus
Zoom Information: Link
Meeting ID: 950 1099 7564
Passcode: chemistry